Established in 1989, Dana Biosphere Reserve is Jordan’s largest biosphere reserve, covering 292 km² of rugged and beautiful landscape along the face of the Great Rift Valley. It sweeps down in a series of mountain ridges, from the 1500m high plateau near Quadesiyya to the desert plains of Wadi Araba. The mountains are cut by many steep-sided wadis, often lined with a lush growth of trees and shrubs. Its geology is as varied as its landscape, switching from limestone to sandstone to granite.

Dana Biosphere Reserve is the only reserve in Jordan that includes the three different bio-geographical zones of the country: Mediterranean, Irano-Turanian, and Sudanian penetration. As such, it is the most diverse nature reserve in the country in terms of habitats and species, hosting several vegetation types, including the Phoenician Juniper, evergreen oak, sand dunes, acacia, and rocky sudanian, among others. It is also home to the southernmost remaining forest community of Cypress Cupressus sempervirens.

More than 891 plant species can be found within the reserve, three of which have only ever been recorded in Dana and nowhere else in the world. Their Latin names include the word ‘Dana’ in them.

Dana supports a wide variety of wildlife, including many rare species of plants and animals. It is home to several globally threatened species of birds and mammals, such as Syrian Serin Serinus syriacus, Lesser Kestrel Falco naumanni, Blanford's Fox Vulpes cana and Nubian Ibex Capra nubiana. The largest breeding colony in the world for Syrian Serin is located in Dana, while the Lesser Kestrel is also found to breed in the area.

The reserve is home to the last and oldest known natural cypress gathering in Jordan. It also contains a large group of plant species, which is 891 species, including 93 rare species and 4 species not recorded elsewhere in Jordan, 75 species confined to the Shobak area and 8 endemic species. 67 species are of natural or nutritional importance, and recently 3 new species were found for science within the boundaries of the reserve. The reserve is also home to a large number of wild animals, many of which are of global importance, including 11 species of mammals, such as Caracal, Blanford's fox, 5 types of reptiles, such as Desert Monitor and the Ground Turtle, in addition to 63 species of birds. It contains the largest known gathering of Syrian Serin in the world. There are 250 bird species recorded in the reserve, 77 of which are nesting, making it one of the most important non-humid areas for bird conservation in the Middle East.

Furthermore, the general number of wild animal species registered in the reserve is 555 species, including 297 species of animals and 258 species of invertebrates.

In 1994, RSCN took pioneering steps in its attempts to conserve the precious biodiversity in Dana, putting together the first protected area management plan in Jordan funded by global environment fund (GEF), making Dana Biosphere Reserve into a model of integrated conservation and socio-economic development. This plan set objectives, strategies, and priorities that ultimately seek to find balance between protecting Dana’s natural wonders and meeting the needs of local people. This strategy was mostly based on the concept of zoning, defining areas where certain activities can or cannot happen, allowing for grazing zones and recreation zones. Following this approach, Dana became the first site in which responsible tourism began taking place.

To date, RSCN has received several global awards for its success in alleviating poverty and creating job opportunities for local communities, in combination with integrating nature conservation.

Major threats to the natural environment of the area still include overgrazing, woodcutting, and hunting, mainly of Ibex and Chukar.